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. 2020 Apr 28;10(5):677. doi: 10.3390/biom10050677

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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DAAM1 (123 kDa) levels in cytosolic and nuclear protein fractions in D-Asp treated rats. (A) Western blot detection of DAAM1 protein in cytosolic and nuclear fractions of D-Asp-treated and control rat testis. To evaluate the purity of the samples, the nuclear protein levels of histone H3 (17 kDa) was detected. (B) The amount of DAAM1 was quantified using ImageJ program and normalized with respect to β-actin (42 kDa). Values represent the means ± S.D. of five samples. * p < 0.05 versus controls.