Table 1.

All the different types of subgroups/groups and related treatment.

Group of 30 Mice	10 Mice for Each Subgroup	Surgery (Splenectomy)	Reinfusion of Autologous Splenic Lymphocytes (SL)	Primary Vaccination	Boost Vaccination	Intravenous Injection of Inactivated Streptococcus pneumoniae	Blood Draw for Antibody Titer
Group A	A1 (Sham positive control)	-	N/A	+			+
	A2 (Negative control)	+	-	+			+
	A3 (Experimental)	+	+	+			+
Group B	B1 (Sham positive control)	-	N/A	+	+		+
	B2 (Negative control)	+	-	+	+		+
	B3 (Experimental)	+	+	+	+		+
Group C	C1 (Sham positive control)	-	N/A	+		+	+
	C2 (Negative control)	+	-	+		+	+
	C3 (Experimental)	+	+	+		+	+

Note: refer to the body of text for details.