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. 2020 Apr 28;10(5):676. doi: 10.3390/biom10050676

Figure 1.

Figure 1

Connexin 46-GFP is overexpressed in MCF-7 cells. MCF-7 breast cancer cells were transfected with human connexin (Cx)-46 gene inserted in pcmV6-AC-GFP plasmid and selected by Neomicyn resistance. (A) Representative of three independent Western blots of MCF-7 and MCF-7Cx46-GFP cell lisates, immunoblots were incubated with anti Cx46, and anti Beta Actin (Santa Cruz Biotechnology; 1:2500). Protein bands were detected using Immobilon Forte western horse-radish protein (HRP) substrate and visualized with a LI-COR CDigit Systems. (B) The graph represents three densitometric independent analyses of the Cx46 and Beta Actin bands obtained using ImageJ software. Graphs were performed using GraphPad. Data are presented as the mean +/− SEM. * p < 0.05 (C) Representative fluorescence images of MCF-7Cx46-GFP cells Nuclei were visualized with Dapi (left), Cx46 was visualized with the GFP tag in the C-terminal portion of Cx46 (middle). Images were obtained using a Nikon Eclipse Ti-U inverted microscope.