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. 2020 Apr 28;10(5):676. doi: 10.3390/biom10050676

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cx46 contained in EVs facilitates the DNA delivery in the recipient cell. A GFP plasmid was incorporated in EV-Cx46 and EVs-sansCx46, and co-cultured with MCF-7 recipient cell for 45 min. Then, the, medium was replacement with EVs-depleted medium. After 24 h and the expression of GFP protein was evaluated by flow cytometry and visualized under fluorescence microscopy. (A) Graph represents the percentage of GFP-positive cells measure by flow cytometry. Data are presented as the mean of three independent experiments ± SEM; **, p < 0.01 and ***, p < 0.001 (B) Representative images of GFP expression in MCF-7 cells at 37 °C and 4 °C conditions.