Table 5.

In silico effect of allelic variants on the miRNAs-binding seed regions. Some SNPs modified the sequence regions of the mRNA causing the deletion or creation of miRNA binding sites. Red line indicates a new miRNA binding site created; red cross indicates that a miRNA binding site were deleted.

Gene	Variant	microRNA	ΔΔG (Kcal/mol)	Duplex SNP-miRNA		Effect
BRCA1	rs8176318 (G/T)	hsa-miR-20a-3p	Wt: 0.00	miRNA:	3′-gaaauuCACGAGU-AUUACGUCa-5′	Created
					|||:||| ||||||
			SNP: −22.70	UTR:	5′-aaccctGTGTTCACAAATGCAGa-3′
	rs12516 (C/T)	hsa-miR-4278	Wt: −18.30	miRNA:	3′-guucccGUUUGGGGGAUc-5′	Created
					:|: |||||||
			SNP: −21.60	UTR:	5′-cttcccTAGCCCCCCTAg-3′
		hsa-miR-637	Wt: −28.30	miRNA:	3′-ugcgucucGGGCUUUCGGGGGUCa-5′	Eliminated
					||| |: ||||X||
			SNP: −16.00	UTR:	5′-taggtcttCCCTAGCCCCCCTAGt-3′
BRCA2	rs15869 (A/C)	hsa-miR-9-5p	Wt: −14.00	miRNA:	3′-ucuuugguuaucUAGCUGUAUga-5′	Created
					|| :|| |
			SNP: −23.20	UTR:	5′-ttatgttgcacaATGAGCAAAga-3′
	c.* 457 (A/C)	hsa-miR-4484	Wt: −11.10	miRNA:	3′-ACCCCGAAGAGGGCGGAAAA-5′	Created
					:| ||||||
			SNP: −23.70	UTR:	5′-TGAAATAAACATACCCTTTT-3′