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. 2020 May 14;10(5):764. doi: 10.3390/biom10050764

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Pharmacokinetic (PK) profiling of antibody carriers in various formats. Five female CD-1 mice each per group were intravenously (i.v.) injected with 6 mg/kg of h38C2 Fab (A), anti-HER2 DVD–Fab (B), anti-HER2 TVD–Fab (C), and anti-HER2 DVD–IgG1 (D). Peripheral blood samples were collected at 5 and 10 min, 1, 6, 12, 24, 48, 96, and 192 h after injection, and used for ELISA to calculate the concentration of antibody carrier in each sample. By using Phoenix WinNonlin PK/PD Modeling and Analysis software, the circulatory half-life (t_½) of antibody carriers was determined. The plots show one representative mouse from each group. All plots show the typical biphasic graph of fast dissemination followed by slow secretion. A two-compartment model was used to determine the t_½ of h38C2 Fab (2.2 ± 0.3 h), anti-HER2 DVD–Fab (5.4 ± 0.5 h), anti-HER2 TVD–Fab antibody (13.3 ± 2.5 h), and anti-HER2 DVD–IgG1 (124 ± 12.5 h).