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. Author manuscript; available in PMC: 2020 Jun 8.
Published in final edited form as: Basic Res Cardiol. 2019 May 31;114(4):28. doi: 10.1007/s00395-019-0737-y

Fig. 1.

Fig. 1

E2F1 is upregulated during heart failure. Male mice aged 10–16 weeks were subjected to sham or myocardial infarction surgery for 1 or 4 week. E2f1 mRNA expression was assessed in whole sham (n = 4) and MI (n = 4) hearts 1 week following MI (a; p = 0.0044 vs Sham). A separate cohort of sham (n = 6) and MI (n = 5) mice was used to spatially determine E2f1 mRNA expression in infarcted (I), non-infarcted (N), and right ventricle (RV) regions of sham and infarcted mice at 1-week post-MI (b). Western blot of E2F1 protein expression and densitometric analysis (c; p = 0.0389 vs Sham). E2f1 mRNA expression in whole Sham and MI hearts 4 weeks following MI (d; p = 0.0133 vs sham). Region-specific expression of E2f1 mRNA in sham (n = 6) and MI (n = 5) hearts 4 week after MI (e). E2F1 immunoblot and densitometric analysis of whole sham and MI hearts 4 weeks following MI (f). An unpaired Student’s t test was used to determine significance between Sham and MI groups. Oneway ANOVA followed by a Newman–Keuls multiple comparison test analysis was used to determine significance between region-specific expression of E2f1