Fig. 10.
E2F1 ablation induces OGA expression in the failing heart. Cardiac tissue harvested from 4-week post-MI E2f1+/+ (n = 13) and E2f1−/− (n = 14) mice were probed for O-GlcNAc enzymes and markers of metabolism. Immunoblot for OGT (a), OGA (b), and protein O-GlcNAcylation (c). Expression of metabolic transcripts via RT-PCR (d) An unpaired Student’s t test was used to determine significance between E2f1+/+ and E2f1−/− groups