Fig. 3.

MAP 3 K2 was the target gene of miR-299-3p and indirectly regulated by MIR205HG. a. Venn data was used to exhibit the common target mRNAs from 3 databases (RNA22, miRmap and miRanda). b. The potential targets of miR-299-3p which was downloaded from starBase website. c. StarBase database showed the binding sites between MAP 3 K2 and miR-299-3p. d. The binding interaction between MAP 3 K2 and miR-299-3p was further assessed by luciferase reporter assay. e. RIP assay was performed to confirm that MIR205HG, miR-299-3p and MAP 3 K2 co-existed in RISCs. f. Western blot analysis was used to determine the protein expression level of MAP 3 K2 in cell lines transfected with sh-MIR205HG#1 and pcDNA3.1/MAP 3 K2. All data were expressed as the mean ± SD. **P < 0.01