Table 1.
PCR primers and probes.
| Organism | Primer/Probe | Sequence (5′–3′) | Concentration (nM) | Reference |
|---|---|---|---|---|
| A. naeslundii | Forward | GAGCACGCCGCTCTGTA | 450 | Ciric et al.16 |
| Reverse | ACCTTGCCGCCTCCGAA | 450 | ||
| Probe | 6FAM-CCTCGTCGCCACGGTGGGTCA-BHQ1 | 150 | ||
| L. casei | Forward | TGGATGCCTTGGCACTAGGA | 900 | Haarman and Knol17 |
| Reverse | AAATCTCCGGATCAAAGCTTACTTAT | 900 | ||
| Probe | 6FAM-TATTAGTTCCGGTCCTTCATC-BHQ1 | 200 | ||
| S. mutans | Forward | CCAGGTCTTGACATCCCGAT | 450 | This study |
| Reverse | CACCTGTCTCCGATGTACCGA | 450 | ||
| Probe | 6FAM-ATTCTTAGAGATAGGAAGTTAC-BHQ1 | 150 | ||
| A. actinomycetemcomitans | Forward | GAACCTTACCTACTCTTGACATCCGAA | 90 | Boutaga et al.18 |
| Reverse | TGCAGCACCTGTCTCAAAGC | 90 | ||
| Probe | 6FAM-AGAACTCAGAGATGGGTTTGTGCCTTAGGG-BBQ | 20 | ||
| P. gingivalis | Forward | GCGCTCAACGTTCAGCC | 90 | Boutaga et al.18 |
| Reverse | CACGAATTCCGCCTGC | 90 | ||
| Probe | Cyan500-CACTGAACTCAAGCCCGGCAGTTTCAA-BBQ | 20 |