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. 2020 Jun 8;21:140. doi: 10.1186/s12931-020-01403-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The concentration of caffeine in serum and expression of A_2AR in lung. Caffeine treatment significantly increased the concentration of caffeine in serum and decreased the A_2AR protein level in the lung. a The concentration of caffeine in serum as determined by ELISA. b The expression of A_2AR in lung tissue as determined by IHC staining (light microscopy, × 200) and western blotting. Scale bars = 100 μm. The values are shown as the mean ± SD; n = 6 mouse/group. ^▲▲P < 0.05 Normoxia (NO) group versus Normoxia +Caffeine (NC) group,^ΨΨP < 0.01 NO group versus Hyperoxia + Caffeine (HC) group, **P < 0.01 HO group versus NO group, ^##P < 0.01 HC group versus HO group., ^&&P < 0.01 HC group versus NC group