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. 2020 May 19;10(5):784. doi: 10.3390/biom10050784

Figure 5.

Figure 5

IEC-derived galectin-9 secretion correlates with IEC/PBMC co-culture cytokine release and IEC-derived TGF-β1. After 24 h IEC/PBMC co-culture, IFNγ, IL-13 and IL-10 concentrations were measured in the basolateral supernatant (Figure 1A and Figure 3). Thereafter, IEC were washed with PBS, the medium was refreshed and IEC incubated for additional 24 h (Figure 1B). After the incubation, the basolateral supernatant was collected and IEC-derived galectin-9 and TGF-β1 were measured (Figure 4). The correlation between IEC-derived galectin-9 and IFNγ (A), IL-13 (B), IL-10 (C) and TGF-β1 (D) release was tested using Spearman’s test (* p < 0.05, *** p < 0.001).