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. 2020 Jun 7;11:2041731420926918. doi: 10.1177/2041731420926918

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Fabrication and characterization of MSC/POC ECM-based TEBs. (a) Pre-osteoclasts (POCs) presented TRAP-positive staining. Scale bars: 200 μm. (b) Schematic illustration of ECM-based TEB fabrication. (c) Quantitative analysis of the total proteins retained on ECM-based TEBs. (d) Biocompatibility analysis of ECM-based TEBs using a mixed lymphocyte reaction (MLR) assay. (e) Surface morphology of ECM-based TEBs under scanning electron microscopy (SEM). Scale bars are 100 μm and 50 μm. (f) Fluorescence staining of DAPI as well as MHC-I and MHC-II. Scale bars: 200 μm. **p < 0.01, *p < 0.05, NS: no significance. (g) Quantification of DNA before and after lyophilization. DNA was nearly completely removed, ***p < 0.001.