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. 2020 Jun 7;11:2041731420926918. doi: 10.1177/2041731420926918

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Proteins released from MSC/POC ECM-based TEBs promoted the migration, adhesion and osteogenic differentiation of MSCs. (a) Extracts from 14-d MSC/POC ECM-based TEBs enhanced the proliferation of MSCs as determined by the CCK–8 assay. (b) DAPI fluorescence of MSC invasion ability by the transwell assay. (c) Scratch area of ECs in different intervention groups at 0 h and 12 h after the scratch was made. Scale bars are 200 μm. (d) Adhesion capacity assessed by the proportion of cells retained in the scaffolds within 6 h. (e) Extracts from MSC/POC ECM-based TEBs enhanced osteogenic gene expression, including Runx2, Col1a1, and Osx, GAPDH was used as a loading control. (f) Representative images of MSCs osteogenic differentiation determined by Alizarin Red S staining and ALP staining. Scale bars are 1 cm. **p < 0.01, *p < 0.05, NS: no significance.