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. 2020 Apr 20;295(23):8096–8105. doi: 10.1074/jbc.AC120.012595

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© 2020 Melick et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 1. — AKAP8L is an mTORC1-interacting protein. A, Raptor interacts with AKAP8L. Shown is co-immunoprecipitation of FLAG-tagged AKAP8L with HA-tagged Raptor. B, co-immunoprecipitation of HA-tagged Raptor with FLAG-tagged AKAP8L. C, co-immunoprecipitation of endogenous AKAP8L with endogenous Raptor. D, co-immunoprecipitation of HA-tagged Raptor with endogenous AKAP8L. E, schematic of the domains of AKAP8L and AKAP8. YG, YG-rich domain; FG, FG repeat region; NLS, nuclear localization sequence. F, mTORC1 interacts with AKAP8L. Shown is co-immunoprecipitation of HA-tagged Raptor and endogenous mTORC1 components with FLAG-tagged AKAP8L. IP, immunoprecipitation; EV, empty vector.