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. 2020 Apr 27;295(23):7941–7957. doi: 10.1074/jbc.RA119.012303

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© 2020 Torii et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 2. — Interactions between CHIKV and ESCRT proteins analyzed by co-IP. HA-tagged host factors and FLAG-tagged CHIKV proteins were exogenously introduced into HEK293T cells. A and B, anti-HA mAb was utilized for IP. Only FLAG-tagged CHIKV protein-expressing plasmid was transfected into the cells as a negative control (NC). A, individual FLAG-tagged CHIKV protein in each fraction was detected by immunoblotting (IB) using the cell lysates. B, individual HA-tagged host factor in each fraction was detected by immunoblotting using the cell lysates. C and D, reciprocally with A and B, anti-FLAG mAb was utilized for IP, and anti-HA mAb was utilized for detection of HA-tagged ESCRT proteins. CHIKV structural proteins (C) and CHIKV nonstructural proteins (D) were examined by IP.