Figure 4.

HGS colocalizes with CHIKV E2 and dsRNA. A, CHIKV (MOI = 10) was inoculated into HGS-Huh-7 cells. At 12 hpi, CHIKV- or mock-infected HGS-Huh-7 cells were fixed and costained for anti-CHIKV E2 (red), anti-HGS (green), and DAPI (blue). B, CHIKV (MOI = 1.0) was inoculated into HGS-Huh-7 cells. At 24 hpi, CHIKV- or mock-infected HGS-Huh-7 cells were fixed and costained for anti-dsRNA (red), anti-HGS (green), and DAPI (blue). The cells were observed by confocal microscopy (Zeiss 780 LSM confocal microscope). Scale bars, 10 μm.