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. 2020 Apr 24;295(23):7865–7876. doi: 10.1074/jbc.RA119.010474

Figure 3.

Figure 3.

Chronic MPP+ treatment induces BAG6 expression. Differentiated SH-SY5Y cells were either untreated (Ctrl) or treated with 250 μm of MPP+ for 2 weeks, and protein expression was analyzed. A, chronic MPP+ treatment induces BAG6 expression and decreases BAG2, BAG5, and PINK1 levels. B, BAG5 protein quantification. *, p = 0.0005. C, BAG2 protein quantification. *, p < 0.0001. D, BAG6 protein quantification. *, p < 0.0001; see also Fig. 1F. E, PINK1-FL. *, p < 0.0001 and PINK1-dN protein quantification, *, p < 0.0001. F, BAG6 mRNA expression normalized to GAPDH, p = 0.0037. Graphs represent means ± S.D.; n = 5 independent experiments for B–E; n = 4 independent experiments for F; Student's two-tailed t test.