Figure 4.

BAG6 knockdown rescues the loss of PINK1 in chronic MPP⁺-treated SH-SY5Y cells. A, control siRNA (siCtrl) and siBAG6 transfected cells were treated with 250 μm MPP⁺ for 2 weeks as described under “Experimental procedures.” B, PINK1-FL quantification. *, p = 0.001 for siCtrl (MPP⁺ versus vehicle (Veh)); †, p = 0.0113 for MPP⁺ (siBAG6 versus siCtrl). C, PINK1-dN quantification. *, p < 0.001 for siCtrl (MPP⁺ versus vehicle); †, p < 0.001 for MPP⁺ (siBAG6 versus siCtrl). Graphs represent means ± S.D.; n = 3 independent experiments; two-way ANOVA with post hoc t tests (see Table 2 for analysis of main factors and interactions).