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AX-024 does not significantly reduce Zap70 phosphorylation. Healthy donor PBMCs were isolated rested overnight and pretreated with 20 μm AX-024 or vehicle prior to stimulation with 20 μg·ml−1 (A) or 10 μg·ml−1 (B) anti-CD3. The cells were gated on live cells, CD3, and CD4 for assessment of P-Zap70. Error bars, S.D.
