Figure 3.

FBXL16 regulates c-myc by antagonizing FBW7. A, Western blot analysis and quantification of c-myc protein level in A549 cells transiently transfected with negative control siRNAs (siCtrl), siRNA targeting FBW7 (siFBW7), siRNA targeting FBXL16 (siFBXL16), or both siFBW7 and siFBXL16. B, 293T cells were transiently co-transfected with HA–c-myc together with either empty plasmid controls, FLAG-FBXL16, HA-FBW7, or both FLAG-FBXL16 and HA-FBW7, followed by Western blot analysis using an anti-FLAG Ab for measuring FBXL16 and an anti-HA Ab for measuring both c-myc and FBW7. Western blots are representative of three independent experiments. Values in graph bars represent mean ± S.E. and statistical significances were determined by Student's t test (*, p < 0.05; **, p < 0.01; ***, p < 0.001).