Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 28;295(23):7970–7980. doi: 10.1074/jbc.RA120.012658

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Morel et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 6. — FBXL16 does not compete with FBW7 for c-myc binding. A, 293T cells were co-transfected with pSG5-FLAG-FBXL16 and either WT, T58/S62AA, T58A, or S62A of HA-tagged c-myc constructs. FLAG-FBXL16 was immunoprecipitated using anti–FLAG-Ab conjugated agarose beads (FLAG-IP), and then Western blot analysis was performed to examine the interactions of FBXL16 with different HA-tagged c-myc protein. B, HA-tagged FBW7 was co-overexpressed with either an empty plasmid or pSG5-FLAG-FBXL16 in 293T cells. HA-FBW7 was then immunoprecipitated using anti–HA-Ab conjugated agarose beads (HA-FBW7 IP). Western blot analysis was then performed to examine the interactions of FBW7 with c-myc.