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. 2020 May 13;10(5):843. doi: 10.3390/ani10050843

Table 6.

Effect of metabolites of foliage of tropical trees on the rumen microbial population and CH4 reduction.

Species Method Treatments Protozoa Bacteria Metanogens References
CFU/mL
Basal diet Sheep fed with concentrate 00:03 138 2930 452 [74]
B. brizantha: Cratylia argente 01:02 207 2530 484
02:01 154 2510 517
Basal diet Sheep fed with concentrate plus S. saponaria (7.71 g crude saponins/lamb/day) in each proportion 00:03 50 3530 493
Cratylia argentea: B. brizantha 01:02 71 4010 697
02:01 91 4180 703
Control RUSITEC 0 6.3 3500 220 [49]
Sapindus saponaria (100 mg fruits/g diet) 120 mg saponins/g fruit 12 2.9 3300 210
Enterolobium cyclocarpum (200 mg fruits/g diet) 19 mg saponins/g fruit 3.8 9.7 3300 210
Pithecellobium saman (200 mg fruit/g diet) 17 mg saponins/g fruit 3.4 9.7 3400 230

RUSITEC: Ruminal simulation technique system; CFU: colony forming units. Protozoa numbers × 103; Bacteria and metanogen numbers × 106; Diet: grass hay (620, 555, 498, 494), Arachis pintoi (248, 222, 194, 195), barley straw (120, 112, 100, 100), and urea (12, 11, 8, 11). Control diet (first value) and (second, third, and fourth value) represents inclusion levels g/kg DM diet ingredients in each tropical fruit tree.