Table 1.

Results of Analysis of Variance: p values of the sources of variation cryoprotectant concentration (C), thawing rate (TR), time of sampling (T) and the relative interactions included in the statistical General Linear Model applied to study chicken sperm quality before and after cryopreservation.

Sperm Parameters 1	C	TR	T	C*TR	C*T	TR*T	C*TR*T
MI	<0.001	<0.001	<0.001	<0.05	<0.001	<0.001	<0.05
TM	<0.001	<0.001	<0.001	<0.05	<0.05	<0.001	<0.05
PM	<0.05	<0.001	<0.001	<0.05	<0.05	<0.001	<0.05
VCL	<0.001	<0.001	<0.001	ns	ns	<0.001	ns
VSL	<0.05	<0.001	<0.001	<0.05	<0.05	<0.001	<0.05
VAP	<0.001	<0.001	<0.001	<0.05	<0.001	<0.001	<0.05
LIN	ns	<0.001	<0.001	ns	ns	<0.001	ns
STR	ns	ns	ns	ns	ns	ns	ns
WOB	ns	<0.001	<0.001	ns	ns	<0.001	ns
ALH	ns	ns	ns	ns	ns	ns	ns
BCF	ns	ns	ns	ns	ns	ns	ns

¹ MI membrane integrity: percentage undamaged membrane spermatozoa; TM total motility: percentage motile spermatozoa; PM progressive motility: spermatozoa swim forward fast in a straight line; VCL: curvilinear velocity; VSL: straight-line velocity; VAP: average path velocity; ALH: amplitude of lateral head displacement; BCF: beat cross frequency; LIN: linearity = (VSL/VCL × 100); STR: straightness = (VSL/VAP × 100); WOB: wobble = (VAP/VCL × 100).