Table 2.
Sperm quality parameters (LSMeans ± SEM) measured in fresh and frozen/thawed semen cryopreserved using different concentration of N-Methylacetamide and different thawing temperatures.
| Sperm Parameters 1 | Fresh Semen | Frozen/Thawed Semen | SEM | |||
|---|---|---|---|---|---|---|
| 6% 5 °C 100s |
6% 38 °C 30s |
9% 5 °C 100s |
9% 38 °C 30s |
|||
| Membrane integrity (%) | 89.9 A | 50.7 B | 22.8 D | 36.6 C | 20.5 D | 1.8 |
| Motility (%) | 87.7 A | 52.3 B | 20.0 D | 35.5 C | 18.1 D | 2.3 |
| Progressive motility (%) | 23.1 A | 11.2 B | 2.2 D | 4.9 C | 1.2 D | 0.9 |
| VCL (µm/s) | 55.5 | 49.1 | 34.9 | 39.3 | 31.7 | 1.6 |
| VSL (µm/s) | 23.6 A | 20.9 B | 11.9 D | 15.3 C | 10.6 D | 0.8 |
| VAP (µm/s) | 36.8 A | 32.0 B | 19.2 D | 23.7 C | 17.5 D | 1.1 |
| LIN (%) | 42.6 | 42.4 | 33.8 | 38.9 | 33.7 | 1.3 |
| STR (%) | 64.2 | 64.9 | 61.6 | 64.3 | 61.0 | 1.2 |
| WOB (%) | 66.3 | 65.0 | 54.7 | 60.3 | 55.2 | 1.1 |
| ALH (µm) | 2.8 | 3.1 | 2.9 | 3.1 | 2.9 | 0.1 |
| BCF (Hz) | 7.5 | 7.2 | 6.5 | 7.3 | 6.7 | 0.4 |
1 Membrane integrity: percentage undamaged membrane spermatozoa; Motility: percentage motile spermatozoa; Progressive motility: spermatozoa swim forward fast in a straight line; VCL: curvilinear velocity; VSL:straight-line velocity; VAP: average path velocity; ALH:amplitude of lateral head displacement; BCF: beat cross frequency; LIN: linearity=(VSL/VCL x 100); STR: straightness= (VSL/VAP x 100); WOB: wobble= (VAP/VCL x 100). A, B, C, D; SEM: standard error of mean. Values within a row with no common superscripts differ significantly at p < 0.001 among treatments.