Table 1. Macromolecule-production information.

Source organism	C. difficile
DNA source	Synthetic codon-optimized sequence
Forward primer CotE-349F	5′-TCCAGGGACCAGCAATGAAAACCCTGAAAGATAGC-3′
Reverse primer CotE-712R	5′-TGAGGAGAAGGCGCGTTAAAACTGGCCATAAATACCTTCC-3′
Cloning vector	pETYSBLLIC3C
Expression vector	pETYSBLLIC3C
Expression host	E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced	MGSSHHHHHHSSGLEVLFQGPAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVDAQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDAALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIRDVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQANLFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINKNGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQANILARTMSIGINDPEVLKEELEGIYGQF