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. 2020 May 29;76(Pt 6):257–262. doi: 10.1107/S2053230X20007025

Table 1. Macromolecule-production information.

Source organism T. virens
DNA source Genomic DNA
Forward primer ATACCATGGCGGCTCCTACTCCTGCG
Reverse primer ATACTCGAGTGGCAAGTTGGCGGGGGCATA
Cloning vector pET-28a(+)
Expression vector pET-28a(+)
Expression host E. coli Rosetta (DE3)
Complete amino-acid sequence of the construct produced MAAPTPADKSMMAAVPEWTITNLKRVCNAGNTSCTWTFGVDTHLATATSCTYVVKANANASQASGGPVTCGPYTITSSWSGQFGPNNGFTTFAVTDFSKKLIVWPAYTDVQVQAGKVVSPNQSYAPANLPLEHHHHHH