Table 2. Crystallization.
Method | Hanging-drop vapour diffusion |
Plate type | 24-well crystallization plate |
Temperature (K) | 289 |
Protein concentration (mg ml−1) | 10 |
Buffer composition of protein solution | 20 mM Tris–HCl pH 8.0, 20 mM NaCl |
Composition of reservoir solution | 1.9 M sodium malonate pH 6.5, 5% glycerol |
Volume and ratio of drop | 5 µl, 1:1 (protein:reservoir solution ratio) |
Volume of reservoir (µl) | 500 |