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. 2020 May 29;76(Pt 6):257–262. doi: 10.1107/S2053230X20007025

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Plate type 24-well crystallization plate
Temperature (K) 289
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 20 mM Tris–HCl pH 8.0, 20 mM NaCl
Composition of reservoir solution 1.9 M sodium malonate pH 6.5, 5% glycerol
Volume and ratio of drop 5 µl, 1:1 (protein:reservoir solution ratio)
Volume of reservoir (µl) 500