Figure 3. AQP3 re-expression had no effect on the expression of markers of keratinocyte proliferation.
Keratinocytes from AQP3-knockout neonatal mice were treated as described in Figure 1. Cells were harvested for qRT-PCR analysis, and the mRNA expression of (a) keratin 5 (K5) and (b) cyclin D1 was analyzed using the ΔΔCt method with the average of GAPDH and RPLP0 expression as the endogenous control. These results are shown as the fold over the vector-infected group and were derived from the analysis of at least three independent experiments; ns: non-significant.