Figure 5. TDP‐43 interacts with HDAC1 and traps HDAC1 in inclusion bodies.

• A
  Left panel: Flag‐tagged full‐length HDAC1 was overexpressed with myc‐tagged TDP‐43 in HEK‐293T cells; the cell lysates were immunoprecipitated for flag and immunoblotted for TDP‐43 and flag. Right panel: myc‐tagged TDP‐43 was overexpressed with flag‐tagged full‐length HDAC1 in HEK‐293T cells; the cell lysates were immunoprecipitated for myc and immunoblotted for flag and TDP‐43.
• B
  Upper left: Flag‐tagged full‐length HDAC1 (b.I) or various truncation mutations (b.II‐IV) were overexpressed with myc‐tagged TDP‐43; the catalytic domain is indicated in red. Lower panel: the Western blotting of cell lysates immunoprecipitated for flag and immunoblotted for TDP‐43.
• C
  Upper panel: Immunoprecipitation of cytosolic HDAC1 and immunoblotting of HDAC1 and TDP‐43 in WT and FTLD‐TDP Tg mice. Lower histogram: Quantification of immunoprecipitation results of HDAC1 and TDP‐43 in WT and Tg mice. N = 5 mice per group, data are presented as mean ± SEM (%), *P = 0.0149, ***P = 0.0003 by t‐test.
• D
  Western blot of HDAC1 and TDP‐43 in urea‐soluble fractions. N = 5 mice per group.

Source data are available online for this figure.