Figure 6.
The cytoprotective properties of V. opulus extracts at IC0 dosages (24 h pre-incubation) against chemical induction (500 µM t-BOOH, 2 h) and oxidative stress analyzed by DCFH-DA on MIN6 cells (A); cells visualized under fluorescent microscope: low fluorescence in control cells, very high fluorescence in cells after incubation with 500 µM t-BOOH, and decreased in cells pre-treated with PRF for 24 h before t-BOOH addition (200 × magnification) (B); MIN6 metabolic activity analyzed with PrestoBlue assay (C). Control cells were not exposed to any compound but the vehicle; values are means ± standard deviations from at least three independent experiments, n ≥ 12; statistical significance was calculated versus cells treated with t-BOOH, ** p ≤ 0.01, *** p ≤ 0.001.