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. 2020 May 12;9(5):415. doi: 10.3390/antiox9050415

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Protective effect of Antirhea borbonica on erythrocytes morphology. Red blood cells morphology was investigated by flow cytometry. (A) Erythrocyte populations were gated according to cell location in a forward scatter (FSC) versus a side scatter (SSC) parameter. R1 and R2 gates represented respectively mature and senescent/aged erythrocytes for RBC (red blood cells); RBC + Ab (in green color); RBC + methylglyoxal (MGO, in red color) and RBC + MGO + Ab. (B) Average percentage of mature erythrocytes (R1) and altered erythrocytes (R2). (C) Average percentage of hemolysis induced by the change in solution tonicity (PBS→NaCl 0.9%). Data are mean ± SEM of five independent experiments. *Effect of MGO (vs. RBC), *** p < 0.001, ** p < 0.01. ^# Effect of Ab (vs. RBC + MGO), ^## p < 0.01, ^# p < 0.05.