. 2020 May 5;9(5):587. doi: 10.3390/foods9050587

Table A2.

Identified compounds found as part of cuticular wax of studied blueberry varieties. ≤LOD—concentration of substance was less than the limit of detection. Concentration of each substance expressed as g 100 g⁻¹ of berry cuticular wax extract. Standard error of measurements was determined to be 3–6% (n = 18).

Compound Class	Substance	RT,min	Patriot	Polaris	Blue Crop	Chandler	Duke	Blue Gold	Chippewa	North Blue
Triterpene	β-sitosterol	21.17	≤LOD	≤LOD	0.074	0.3	≤LOD	≤LOD	0.235	0.3
	β-amyrin	21.38	1.795	1.914	≤LOD	1.865	3.195	≤LOD	2.576	1.439
	α-amyrin	21.83	9.519	11.066	6.76	4.211	3.842	1.625	6.374	2.351
	Lupeol	22.04	≤LOD	10.196	9.21	1.418	1.017	11.2	≤LOD	≤LOD
	Lanosterol	22.14	≤LOD	≤LOD	0.508	0.35	≤LOD	≤LOD	≤LOD	≤LOD
	Uvaol	23.83	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD
	Erythrodiol	24.43	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD
	Betulinic acid	25.13	≤LOD	1.994	0.244	5.065	8.33	6.695	1.964	2.165
	Ursolic acid	25.46	4.241	9.304	2.116	2.938	3.356	3.231	0.459	5.115
	Ursolic aldehyde	26.2	≤LOD	≤LOD	≤LOD	0.007	≤LOD	0.012	≤LOD	≤LOD
	Betulonic acid	26.5	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD
	Subtotal		15.555	34.474	18.912	16.154	19.74	22.763	11.608	11.37
Alkane	C₂₀	4.37	0.05	0.082	≤LOD	0.05	0.05	≤LOD	0	0.05
	C₂₃	7.34	0.05	0.074	≤LOD	0.05	0.05	≤LOD	0.05	≤LOD
	C₂₅	10.03	0.05	0.057	0.074	0.057	0.057	≤LOD	0.057	0.057
	C₂₇	13.24	0.05	0.074	0.093	0.064	0.05	0.075	0.778	0.157
	C₂₈	14.51	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD
	C₂₉	15.65	1.138	0.691	0.219	0.691	0.676	0.648	0.453	1.226
	C₃₀	16.64	0.043	0.1	0.075	0.057	0.05	0.075	0.146	0.093
	C₃₁	17.64	0.937	0.758	0.075	0.423	0.138	0.075	0.08	0.596
	C₃₃	19.9	0.079	≤LOD	≤LOD	≤LOD	≤LOD	0.075	0.044	0.07
	Subtotal		2.397	1.836	0.536	1.392	1.071	0.948	1.608	2.249
Fatty acid	Palmitic acid	5.23	0.4	0.3	0.4	0.3	0.4	0.3	0.5	0.126
	Stearic acid	6.74	0.25	0.3	0.25	0.3	0.25	0.3	0.25	0.13
	Nonadecanoic acid	7.81	0.769	0.684	0.84	1.084	1.349	1.362	0.195	0.34
	Eicosanoic acid	9.12	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD
	Behenic acid	12.4	0.806	1.304	0.34	1.213	0.457	0.307	1.809	0.74
	Lignoceric acid	14.93	1.076	1.013	0.98	2.466	1.106	0.87	1.738	≤LOD
	Hexacosanoic acid	17.03	0.241	1.266	0.336	4.402	0.833	0.671	1.7	0.607
	Octacosanoic acid	18.83	0.75	2.068	1.062	1.518	0.993	3.26	3.249	1.015
	Nonacosanoic acid	20.85	1.549	2.205	1.2	1.861	2.197	1.56	2.687	0.9
	Triacontanoic acid	21.54	6.958	6.005	4	1.8	3.2	3.5	≤LOD	9.558
	Subtotal		12.799	15.145	9.408	14.944	10.785	12.13	12.128	13.416
Acetate	Hexacosyl acetate	14.64	0.974	1.013	1.542	1.927	1.294	0.776	1.738	0.549
Alcohol	Phytol	6.18	0.162	0.195	0.242	0.531	0.352	0.335	0.291	0.189
	Docosanol	10.93	0.129	0.068	0.122	0.13	0.073	0.128	0.137	0.247
	Tetracosanol	13.88	0.374	0.477	0.094	0.34	0.206	0.167	0.503	0.81
	9-C₂₇-ol	15.37	2.407	0.429	0.832	4.373	1.193	1.951	1.414	1.84
	1-Hexacosanol	16.12	≤LOD	≤LOD	≤LOD	0.001	≤LOD	≤LOD	≤LOD	≤LOD
	9-C₂₈-ol + 10-C₂₈-ol	16.37	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	0.037	≤LOD	≤LOD
	10-C₂₉-ol	17.27	≤LOD	0.259	≤LOD	0.107	0.014	0.24	0.128	0.062
	8,9-C₂₇-diol	18.56	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD
	11-C₃₁-ol	19.42	0.007	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD
	1-Triacontanol	20.48	1.334	0.869	1.021	1.427	1.237	2.064	2.473	1.52
	1-Dotriacontanol	23.49	≤LOD	≤LOD	0.184	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD
	Subtotal		4.413	2.297	2.495	6.909	3.075	4.922	4.946	4.668
Aldehyde	Hexacosanal	12.33	0.103	0.17	0.078	0.14	0.015	0.137	0.216	0.119
	Heptacosanal	13.75	0.509	0.605	0.137	0.123	0.269	0.322	0.494	1.234
	Octacosanal	14.4	0.292	0.176	0.132	0.333	0.091	0.165	0.365	0.574
	Triacontanal	17.09	0.566	0.963	0.683	5.581	2.033	0.614	1.7	2.74
	Dotriacontanal	19.31	0.151	2.357	2.219	2.459	2.99	3.074	4.66	1.395
	Subtotal		1.621	4.271	3.249	8.636	5.398	4.312	7.435	6.062
Carbohydrate	beta.-D-Allopyranose	4.85	0.011	≤LOD	≤LOD	0.025	0.015	0.014	0.012	0.017
	Myo-Inositol	5.8	≤LOD	≤LOD	0.006	0.021	0.023	0.01	≤LOD	≤LOD
	Carbohydrate	9.55	0.195	0.183	0.121	0.397	0.165	≤LOD	0.13	0.174
	Subtotal		0.206	0.183	0.127	0.443	0.203	0.024	0.142	0.191
Sesquiterpene	Abscisic acid	6.61	0.048	0.075	0.045	0.056	0.082	0.067	0.037	0.069
Ester	Octacosanoate	19.17	0.75	3.068	1.062	1.518	0.993	3.26	5.249	1.015
Ketone	Nonacosane-8,10-dione	18.65	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD
Ketone	Hentriacontane-10,12-dione	21.27	2.567	2.918	1.334	3.441	2.297	0.583	3.858	6.001
	Subtotal		2.567	2.918	1.334	3.441	2.297	0.583	3.858	6.001
Phenolic acid	Syringic acid	4.3	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD	≤LOD
	p-Coumaric acid	4.65	0.031	0.004	0.024	0.042	0.034	0.011	0.021	0.02
	Methyl caffeate	5.11	0.055	0.107	0.025	0.086	0.029	0.068	0.083	0.009
	Ferulic acid	5.65	≤LOD	0.002	≤LOD	≤LOD	0.015	≤LOD	≤LOD	≤LOD
	Subtotal		0.086	0.113	0.049	0.128	0.078	0.079	0.104	0.029
Tocopherols	alpha.-Tocopherol	18.32	0.137	0.218	0.154	0.192	0.234	0.284	0.2	≤LOD
	Total		41.553	65.611	38.913	55.74	45.25	50.148	49.053	45.619