Figure 1.

Microtiter ELISA plate shows the patterns of biofilm formation by MDR-CoNS strains. The strains listed in Table 1 were cultured overnight in in a 96-well flat-bottom polystyrene microtiter plate in trypticase soy broth (TSB) medium supplemented with 0.25% glucose. The cells that adhered to the plate after washing with phosphate-buffered saline (PBS) were then visualized by staining with crystal violet and solubilized in 1% w/v SDS. The optical density was determined at optical density 570 nm (OD_{570 nm}) and the assay was performed in triplicate a long with the standard deviation (±SD).