Figure 3.
The effect of fucoidan on H2O2-induced oxidative stress in Vero cells. (A) The protective effect of fucoidan against H2O2-induced cell death in Vero cells; (B) the intracellular reactive oxygen species (ROS) scavenging effect of fucoidan in H2O2-stimulated Vero cells; (C) the protective effect of fucoidan against H2O2-induced apoptosis in Vero cells. The intracellular ROS level was determined by DCF-DA assay and the cell viability was evaluated by MTT assay. The apoptotic body formation was observed under a fluorescence microscope after Hoechst 33342 staining. Apoptosis levels were measured using Image J software. The experiments were conducted in triplicate, and the data are expressed as the mean ± SE. * p < 0.05, ** p < 0.01 as compared to the H2O2-treated group and ## p < 0.01 as compared to the control group.