Figure 2.

Taurisolo shifts mice liver metabolism toward mitochondrial respiration. (A–F) Metabolomic profiling of liver biopsies excised from mice fed High Fat Diet (HFD) and receiving Taurisolo (black dots) or Placebo (white dots) for 4 weeks (see also Table 2). Each dot represents the mass spectrometry intensity of the indicated metabolites as measured by FT-ICR-MS. In (B,D), mass spectrometry data for glucose and cholesterol were confirmed by measuring their intracellular concentration (using enzymatic reactions) in Taurisolo (black bars) or Placebo (gray bars) groups. (Data are representative of n = 6 triplicated measurements, shown is mean ± s.e.m. Two-way ANOVA and Bonferroni post-test analysis were performed; *** p < 0.001;). (G) The fluorescent emission of Mitotracker CMX-ROS (red channel) was here used to show the increase in mitochondrial membrane potential induced by Taurisolo in murine livers. Liver biopsies collected from mice treated with Taurisolo or placebo were incubated ex-vivo with Mitotracker CMX-ROS. UPPER PANEL: A faint fluorescence emission of the probe (red channel) is detectable in the hepatocytes of the placebo group. LOWER PANEL: Increased fluorescence emission of Mitotracker CMX-ROS in hepatocytes of the Taurisolo group. DAPI (blue channel) stains cell nuclei. In G, scale bars correspond to 50 μm.