Table 2.
Chemical composition of essential oils hydrodistillated from Zanthoxylum leprieurii leaves. Each analysis was realized in triplicate.
| Leaves | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| N° | Compounds | Cas Number | Identification | Ria | Rib | May | June | July | Aug | Sep | Oct | Nov |
| 1 | α-pinene | 80-56-8 | MS, RI, STD | 931 | 929 | 2.48 ± 0.13 | 8.45 ± 0.57 | 0.30 ± 0.11 | 0.60 ± 0.35 | 0.70 ± 0.15 | 0.50 ± 0.27 | 0.30 ± 0.17 |
| 2 | ß-myrcene | 123-35-3 | MS, RI, STD | 987 | 988 | 0.71 ± 0.03 | - | 5.20 ± 0.21 | 0.90 ± 0.15 | 3.40 ± 0.37 | 0.4 ± 0.31 | 0.70 ± 0.09 |
| 3 | p-cymene | 25155-15-1 | MS, RI, STD | 1022 | 1022 | 1.48 ± 0.03 | - | 0.10 ± 0.01 | 0.30 ± 0.03 | - | 0.40 ± 0.57 | - |
| 4 | limonene | 138-86-3 | MS, RI, STD | 1023 | 1027 | - | 0.90 ± 0.29 | 0.40 ± 0.28 | - | - | - | - |
| 5 | (E)-ß-ocimene | 13877-91-3 | MS, RI, STD | 1041 | 1046 | 23.57 ± 047 | 2.60 ± 0.99 | 3.90 ± 0.84 | 2.50 ± 0.04 | 1.30 ± 0.50 | 2.30 ± 1.91 | - |
| 6 | γ-terpinene | 99-85-4 | MS, RI, STD | 1060 | 1057 | 0.28 ± 0.03 | - | - | - | - | - | - |
| 7 | linalool | 78-70-6 | MS, RI, STD | 1094 | 1098 | 6.44 ± 0.13 | 0.50 ± 0.34 | 1.50 ± 0.55 | 8.50 ± 0.09 | 1.70 ± 0.23 | 4.30 ± 0.95 | 3.20 ± 0.09 |
| 8 | alloocimene | 673-84-7 | MS, RI | 1128 | 1129 | 1.22 ± 0.04 | 0.40 ± 0.13 | 0.20 ± 0.10 | - | - | - | - |
| 9 | terpineol | 98-55-5 | MS, RI, STD | 1190 | 1191 | - | - | 0.20 ± 0.03 | - | - | - | - |
| 10 | decanal | 112-31-2 | MS, RI, STD | 1191 | 1204 | - | - | 1.50 ± 0.52 | - | - | - | - |
| 11 | citronellol | 106-22-9 | MS, RI, STD | 1225 | 1227 | - | - | 2.00 ± 0.48 | - | 1.50 ± 0.27 | - | 0.30 ± 0.02 |
| 12 | geraniol | 106-24-1 | MS, RI, STD | 1250 | 1253 | - | - | 1.40 ± 0.53 | - | 1.00 ± 0.74 | - | - |
| 13 | decyl alcohol | 112-30-1 | MS, RI, STD | 1262 | 1263 | - | - | 1.80 ± 0.92 | - | - | - | - |
| 14 | thymol | 89-83-8 | MS, RI, STD | 1286 | 1291 | - | 2.20 ± 0.96 | 3.10 ± 0.43 | 13.30 ± 0.31 | 4.10 ± 0.22 | - | - |
| 15 | undecan-2-one | 112-12-9 | MS, RI, STD | 1288 | 1293 | 8.51 ± 0.35 | 1.90 ± 0.50 | 1.20 ± 0.03 | 8.40 ± 0.12 | 2.70 ± 0.56 | 3.20 ± 1.06 | 2.10 ± 0.98 |
| 16 | undecan-2-ol | 1653-30-1 | MS, RI, STD | 1298 | 1300 | - | - | 0.20 ± 0.04 | 0.40 ± 0.04 | - | - | - |
| 17 | methyl nerate | 1862-61-9 | MS, RI, STD | 1319 | 1323 | - | - | 2.20 ± 0.74 | - | 0.80 ± 0.44 | - | - |
| 18 | δ-elemene | 20307-84-0 | MS, RI | 1334 | 1339 | - | 1.20 ± 0.69 | 0.20 ± 0.00 | - | - | - | - |
| 19 | α-copaene | 3856-25-5 | MS, RI, STD | 1376 | 1378 | - | 0.60 ± 0.23 | 0.30 ± 0.08 | - | 0.60 ± 0.11 | 0.20 ± 0.07 | 0.40 ± 0.04 |
| 20 | ß-elemene | 515-13-9 | MS, RI | 1388 | 1394 | 3.92 ± 0.06 | - | 2.70 ± 0.83 | 4.20 ± 0.07 | 5.90 ± 0.26 | 2.30 ± 0.10 | 2.90 ± 0.20 |
| 21 | β-caryophyllene | 87-44-5 | MS, RI, STD | 1419 | 1423 | 13.51 ± 0.11 | 18.90 ± 0.48 | 15.60 ± 0.54 | 13.70 ± 0.1 | 19.85 ± 0.82 | 7.00 ± 1.02 | 8.60 ± 0.62 |
| 22 | cadina-4(14),5-diene | 54324-03-7 | MS, RI | 1430 | 1433 | 1.83 ± 0.02 | 0.90 ± 0.26 | 0.70 ± 0.16 | 0.30 ± 0.02 | 2.40 ± 0.81 | 1.70 ± 0.26 | 1.50 ± 0.19 |
| 23 | γ-elemene | 3242-08-8 | MS, RI | 1432 | 1435 | 1.19 ± 0.01 | 4.40 ± 0.45 | 1.10 ± 0.21 | 1.10 ± 0.04 | 3.10 ± 0.06 | 0.8 ± 0.12 | 1.10 ± 0.01 |
| 24 | α-humulene | 6753-98-6 | MS, RI, STD | 1456 | 1457 | 3.92 ± 0.02 | 6.70 ± 0.62 | 4.50 ± 0.98 | 3.60 ± 0.04 | 6.10 ± 0.27 | 4.10 ± 0.58 | 4.20 ± 0.32 |
| 25 | alloaromadendrene | 25246-27-9 | MS, RI | 1457 | 1465 | - | 0.50 ± 0.21 | 0.30 ± 0.06 | - | 0.50 ± 0.11 | 0.40 ± 0.06 | 0.20 ± 0.01 |
| 26 | germacrene D | 23986-74-5 | MS, RI, STD | 1482 | 1485 | 1.96 ± 0.17 | 0.90 ± 0.50 | 0.40 ± 0.10 | - | 1.60 ± 0.40 | 1.40 ± 0.21 | 1.00 ± 0.14 |
| 27 | ß-ionone | 14901-07-6 | MS, RI, STD | 1482 | 1488 | - | - | 0.40 ± 0.12 | - | - | - | 0.20 ± 0.05 |
| 28 | ß-selinene | 17066-67-0 | MS, RI | 1483 | 1490 | 0.32 ± 0.01 | 1.10 ± 0.13 | 0.70 ± 0.14 | 0.80 ± 0.01 | 1.60 ± 0.30 | 0.20 ± 0.21 | 0.50 ± 0.05 |
| 29 | tridecan-2-one | 593-08-8 | MS, RI, STD | 1487 | 1495 | 18.74 ± 0.57 | 9.00 ± 0.02 | 22.50 ± 0.98 | 30.20 ± 0.39 | 15.80 ± 0.84 | 36.80 ± 0.06 | 33.70 ± 0.36 |
| 30 | selina-4(14),7(11)-diene | 515-17-3 | MS, RI | 1495 | 1498 | - | 1.30 ± 0.31 | 0.80 ± 0.15 | 1.50 ± 0.90 | 2.90 ± 0.86 | - | - |
| 31 | tridecan-2-ol | 1653-31-2 | MS, RI, STD | 1495 | 1501 | - | 2.00 ± 0.82 | 1.70 ± 1.17 | - | 2.30 ± 0.29 | 2.40 ± 0.80 | 3.20 ± 0.33 |
| 32 | (3E,6E)-α-farnesene | 502-61-4 | MS, RI | 1499 | 1509 | 3.22 ± 0.29 | 0.60 ± 0.21 | 1.20 ± 0.34 | 0.90 ± 0.07 | 2.50 ± 0.97 | 9.10 ± 0.82 | 4.60 ± 0.55 |
| 33 | γ-cadinene | 39029-4-9 | MS, RI | 1513 | 1517 | 0.42 ± 0.06 | 1.10 ± 0.36 | 0.60 ± 0.08 | 0.20 ± 0.04 | 1.00 ± 0.21 | 1.20 ± 0.26 | 1.30 ± 0.02 |
| 34 | δ-cadinene | 483-76-1 | MS, RI | 1524 | 1526 | 2.25 ± 0.09 | 4.20 ± 1.01 | 2.20 ± 0.09 | 1.00 ± 0.02 | 4.30 ± 0.13 | 3.40 ± 0.49 | 4.30 ± 0.33 |
| 35 | elemol | 639-99-6 | MS, RI, STD | 1547 | 1552 | 0.28 ± 0.02 | 0.60 ± 0.22 | 0.20 ± 0.01 | 0.30 ± 0.01 | 0.30 ± 0.05 | 0.40 ± 0.03 | 1.20 ± 0.09 |
| 36 | nerolidol | 7212-44-4 | MS, RI, STD | 1557 | 1564 | - | 1.50 ± 0.30 | 1.10 ± 0.39 | 1.00 ± 0.03 | 1.60 ± 0.49 | 4.80 ± 0.22 | 3.40 ± 0.32 |
| 37 | dendrolasin | 23262-34-2 | MS, RI | 1576 | 1580 | 1.79 ± 0.08 | 8.60 ± 0.95 | 9.40 ± 0.90 | 4.00 ± 0.06 | 7.60 ± 0.09 | 10.60 ± 0.44 | 16.40 ± 0.85 |
| 38 | spathulenol | 6750-60-3 | MS, RI | 1578 | 1582 | - | 1.50 ± 0.46 | 1.10 ± 0.03 | - | - | - | 0.40 ± 0.00 |
| 39 | caryophyllene oxide | 1139-30-6 | MS, RI, STD | 1583 | 1588 | - | 6.00 ± 0.83 | 5.70 ± 0.79 | - | - | - | - |
| 40 | ζ-cadinol | 5937-11-1 | MS, RI | 1639 | 1645 | - | 1.10 ± 0.08 | 0.60 ± 0.03 | - | 0.70 ± 0.21 | 0.60 ± 0.15 | 0.80 ± 0.04 |
| 41 | α-cadinol | 481-34-5 | MS, RI | 1650 | 1659 | 0.28 ± 0.03 | 1.30 ± 0.11 | 0.70 ± 0.01 | - | 1.10 ± 0.44 | 0.80 ± 0.23 | 0.90 ± 0.09 |
| 42 | pentadecanal | 2765-11-9 | MS, RI | 1713 | 1715 | - | 2.10 ± 0.28 | - | - | - | - | 1.60 ± 0.79 |
| Monoterpene hydrocarbons (%) | 29.74 | 12.85 | 10.10 | 4.30 | 5.40 | 3.60 | 1.00 | |||||
| Oxygenated monoterpene (%) | 6.44 | 2.70 | 11.50 | 21.80 | 8.20 | 4.30 | 3.50 | |||||
| Sesquiterpene hydrocarbons (%) | 32.54 | 48.10 | 30.90 | 27.30 | 52.35 | 31.80 | 30.60 | |||||
| Oxygenated sesquiterpenes (%) | 2.35 | 22.70 | 18.10 | 5.30 | 12.10 | 17.20 | 25.40 | |||||
| Others (%) | 27.25 | 13.10 | 28.90 | 39.00 | 20.80 | 42.40 | 38.50 | |||||
| Identified compounds (%) | 98.32 | 99.45 | 99.50 | 97.70 | 98.85 | 99.30 | 99.00 | |||||
| Yield (%) | 0.02 | 0.02 | 0.03 | 0.04 | 0.02 | 0.02 | 0.02 | |||||
Identification methods: RIa, theoretical kovats indices (Pubchem and NIST); RIb, calculated kovats indices; MS, mass spectra comparison with PAL 600®libraries; STD, retention time and mass spectra comparison with commercially available standards; RI, retention index comparison with the literature; CAS number; -, Under perception threshold.