Table 2.
Features of primers for qRT-PCR of ten selected genes. Table lists attributes of primers used for qRT-PCR validation of ten selected lncRNAs.
| Gene Symbol | Gene ID (ENSEMBL) | Primer Forward (5′ → 3′) |
Primer Reverse (5′ → 3′) |
Length (bp) | TM (°C) |
|---|---|---|---|---|---|
| CLIP1 | ENSG00000130779 | TGGCGTGGAGTTAGATGAGC | GGTGTAGTGGAAGGGAAGCC | 138 | 62 |
| SRSF5 | ENSG00000100650 | CCCGTGCCTGAGAAGAGC | TGCCACTGTCAACTGATCTGG | 115 | 62 |
| APOLD1 | ENSG00000178878 | GGACCAGATGCGAGAGATCC | CACGTGAGCCAAAGAAGACG | 146 | 62 |
| LYN | ENSG00000254087 | AGTCTGATGTGTGGTCCTTTGG | GCTCATCTGGGCAGTTCTCC | 147 | 62.5 |
| RASGRP3 | ENSG00000152689 | ACTGTGCGGGATTTCTCTGG | CCCATGACCACTGCTCAAGG | 146 | 62.5 |
| ITGA4 | ENSG00000115232 | GAAAGAATCCCGGCCAGACG | GGCTGTCTGGAAAGTGTGACC | 124 | 63.5 |
| PDK4 | ENSG00000004799 | TTTCTACTCGGATGCTGATGAACC | GCATCTTGGACCACTGCTACC | 121 | 63 |
| SGK1 | ENSG00000118515 | AAATGTGAGTGGGCCCAACG | CTTGACGCTGGCTGTGACG | 115 | 63.5 |
| PIK3AP1 | ENSG00000155629 | GAAGCTGGGCATTGTCAACG | CTCTCTGTCTTCGGGTGATGC | 143 | 63 |
| SPP1 | ENSG00000118785 | CCGAGGTGATAGTGTGGTTTATGG | GGTGATGTCCTCGTCTGTAGC | 97 | 63 |