Figure 4.

Treatment of lens epithelial cells with H₂O₂. (A) and (B) refer to H₂O₂ removal and viability assay in HLEC, respectively. (C) and (D) refer to H₂O₂ removal and viability assay in bovine lens epithelial cells (BLEC), respectively. Panel (A): HLEC at 70% confluence (2.5 × 10⁶ cells) were incubated in Minimum Essential Medium (7 mL) at 37 °C; at the indicated times cells were supplemented with 50 (gray), 100 (green) or 150 (red) µM H₂O₂ and the concentration of the peroxide was measured as described in Section 2.4. C: BLEC at 70% confluence (1.0 × 10⁶ cells) were incubated in HBSS (7 mL) at 37 °C; at the indicated times, cells were supplemented with 50 (gray), 100 (green) or 150 (red) µM H₂O₂ and the concentration of the peroxide was measured as described in Section 2.4. (C) and (D): Cell viability was evaluated by MTT assay (see Section 2.3) at the end of overall oxidative treatment at the indicated concentrations of H₂O₂. The values represent the mean ± SEM of at least three independent experiments. Significance was evaluated with respect to untreated cells (** p < 0.01, *** p < 0.001, **** p < 0.0001).