Table 1.
Retention time (Rt), wavelengths of maximum absorption in the visible region (λmax), mass spectrometric data, tentative identification, and quantification (mg/g of extract) of the phenolic compounds in Ocimum basilicum ’Cinnamon’ and Ocimum × citriodorum hydroethanolic extracts and infusion preparations.
| Peak | Rt (min) | λmax (nm) | [M − H]− (m/z) | MS2 (m/z) | Tentative Identification | Quantification (mg/g of Extract) | |||
|---|---|---|---|---|---|---|---|---|---|
| Ocimum basilicum cv. ’Cinnamon’ | Ocimum × citriodorum | ||||||||
| EtOH:H2O | Infusion | EtOH:H2O | Infusion | ||||||
| 1 | 4.81 | 326 | 341 | 179(100),161(62),135(34) | Caffeic acid hexoside A | tr | tr | tr | tr |
| 2 | 9.89 | 323 | 179 | 135(100) | Caffeic acid A | 1.194 ± 0.002 c | 0.63 ± 0.02 d | 1.41 ± 0.03 b | 3.1 ± 0.1 a |
| 3 | 13.54 | 327 | 473 | 311(100),293(98),179(8),149(5),135(5) | Chicoric acid A | 0.51 ± 0.03 d | 0.61 ± 0.01 c | 0.64 ± 0.01 b | 1.09 ± 0.05 a |
| 4 | 15.88 | 350 | 595 | 463(25),301(100) | Quercetin-O-pentoside-O-hexoside B | 2.07 ± 0.06 a | 1.54 ± 0.02 b | nd | nd |
| 5 | 17.63 | 355 | 609 | 301(100) | Quercetin-3-O-rutinoside B | 4.53 ± 0.01 a | 3.24 ± 0.04 b | 1.525 ± 0.003 d | 2.85 ± 0.02 c |
| 6 | 18.21 | 340 | 717 | 537(40),519(100),493(15),359(10),339(8),321(5) | Salvianolic acid B isomer 1 C | nd | nd | 2.23 ± 0.04 b | 2.47 ± 0.03 a |
| 7 | 18.53 | 341 | 463 | 301(100) | Quercetin-3-O-glucoside D | 3.405 ± 0.003 a | 2.22 ± 0.04 d | 2.91 ± 0.08 b | 2.43 ± 0.02 c |
| 8 | 20.05 | 337 | 549 | 505(5),463(28),301(100) | Quercetin-O-malonyl-hexoside B | 3.4 ± 0.1 a | 2.82 ± 0.03 b | 1.87 ± 0.02 d | 2.11 ± 0.03 c |
| 9 | 20.45 | 337 | 549 | 505(6),463(48),301(100) | Quercetin-O-malonyl-hexoside B | nd | nd | 1.58 ± 0.02 b | 1.84 ± 0.05 b |
| 10 | 21.75 | 339 | 359 | 197(25),179(41),161(100),135(5) | Rosmarinic acid C | 77 ± 1 a | 41.0 ± 0.2 d | 50 ± 1 c | 59.1 ± 0.3 b |
| 11 | 25.79 | 329 | 717 | 537(5),519(100),493(5),339(5),321(7),295(5) | Salvianolic acid B isomer 2 C | 5.2 ± 0.1 c | 7.3 ± 0.4 a | nd | 6.9 ± 0.1 b |
| 12 | 30.44 | 284/329 | 537 | 493(100),439(5),359(62),197(5),179(10),161(15) | Lithospermic acid A C | 7.11 ± 0.02 a | 2.82 ± 0.06 b | nd | nd |
| Total Phenolic Acids | 91 ± 1 a | 52.4 ± 0.4 d | 55 ± 1 c | 72.7 ± 0.3 b | |||||
| Total Flavonoids | 13.4 ± 0.2 a | 9.8 ± 0.1 b | 7.9 ± 0.1 d | 9.2 ± 0.1 c | |||||
| Total Phenolic Compounds | 105 ± 1 a | 62.2 ± 0.1 c | 63 ± 2 c | 81.9 ± 0.2 b | |||||
nd—not detected. tr—traces. Standard calibration curves used for compounds’ quantification: A—caffeic acid (y = 388345x + 406369, R2 = 0.9939, limit of detection (LOD) and limit of quantitation (LOQ) = 0.78 and 1.97 µg/mL, respectively); B—quercetin-3-O-rutinoside (y = 13343x + 7675, R2 = 0.9998, LOD and LOQ = 0.18 and 0.65, respectively); C—rosmarinic acid (y = 191291x – 652903, R2 = 0.999, LOD and LOQ = 0.15 and 0.68, respectively); D—quercetin-3-O-glucoside (y = 34843x – 160173, R2 = 0.9998, LOD and LOQ = 0.21 and 0.71, respectively). Different letters (lowercase letters) correspond to significant differences (p < 0.05).