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. 2020 May 6;9(5):388. doi: 10.3390/antiox9050388

Figure 1.

Figure 1

Detection of authentic 3-NT standards by HPLC/ECD. (A) The coulometric signal for 3-NT increased for the applied potentials from 650 to 800 mV as shown by the representative chromatograms. (B) The sensitivity of the HPLC/ECD analysis was good and a concentration of 25 nM 3-NT was easily detectable as shown by the representative chromatograms. (C) The calibration curve was highly linear over a concentration range of 10–500 nM. Analysis was carried on with 20 µL of sample at 27 °C with isocratic elution using a flow of 1 mL/min and a mobile phase consisting of 26.3 mM sodium citrate and 10.9 mM sodium at pH 4.75 with 2.8 v/v% methanol (RT of 3-NT was observed at 6.2 min).