Figure 3.
Evaluation of the changes in the mitochondrial membrane potential (a) and intracellular calcium levels (b) produced after incubation for 24 h with bioaccessible fractions obtained from Brassicaceae microgreens (diluted 1:10 v/v in DMEM) in Caco-2 cells. Results are expressed as mean ± standard deviation (n = 4) in two independent assays. Different lowercase letters (a–c) indicate statistically significant differences (p < 0.05) among samples. Positive control (5-FU) produced a marked mitochondrial depolarization (6-fold) and raise of intracellular Ca2+ levels (64%) compared to control (untreated cells).