KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| See Table S1 for Antibody information | N/A | N/A |
| Biological Samples | ||
| Pancreatic cancer PDX: MSK-PR07 | MSKCC/This paper | N/A |
| Pancreatic cancer PDX: MSK-PR05 | MSKCC/This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Trametinib (in vitro) | Selleck chemicals | Cat#S2673 |
| Palbociclib (in vitro) | Selleck chemicals | Cat#S1116 |
| Trametinib (in vivo) | LC Laboratories | Cat#T-8123 |
| Palbociclib (in vivo) | LC Laboratories | Cat#P-7744 |
| Gemcitabine | MSKCC Pharmacy | Cas#:122111-03-9 |
| 14C-Gemcitabine | Moravek Biochemicals | Cat#MC 2246 |
| Recombinant mouse IL-2 | Biolegend | Cat#575402 |
| Recombinant human IL-7 | Peprotech | Cat#200-07 |
| Recombinant human IL-15 | Peprotech | Cat#200-15 |
| Matrigel | Corning | Cat#354230 |
| Solvable | Perkin Elmer | Cat#6NE9100 |
| Ultima Gold | Perkin Elmer | Cat#6013321 |
| Collagenase type V | Sigma | Cat#C9263 |
| Culturex reduced growth factor basement membrane extract | R&D systems | Cat#3533-005-02 |
| B27 supplement | Invitrogen | Cat#12634-028 |
| hEGF | Peprotech | Cat#PMG8034 |
| mNoggin | Peprotech | Cat#250-38 |
| hFGF10 | Peprotech | Cat#100-26 |
| hLeu-Gastrin I | Sigma | Cat#G9145 |
| N-acetylcysteine | Sigma | Cat#A9165 |
| Nicotinamide | Sigma | Cat#N0636 |
| Dynabeads Mouse T-Activator CD3/CD28 | Thermo Fisher Scientific | Cat#11452D |
| PMA | Sigma | Cat#P8139 |
| Ionomycin | Stem cell technologies | Cat#73724 |
| GolgiStop | BD bioscience | Cat#554724 |
| Trizol | Invitrogen | Cat#15596026 |
| Biotinylated tomato lectin | Vector Laboratories | Cat#B1175 |
| FITC-dextran (MW 2,000,000) | Sigma-Aldrich | Cat#52471 |
| Pimonidazole | Hypoxyprobe | Cat#HP1-1000Kit |
| Critical Commercial Assays | ||
| Human Cytokine/Chemokine Array 42-Plex Discovery Assay | Eve Technologies | Cat#HD42 |
| Mouse Cytokine/Chemokine Array 31-Plex | Eve Technologies | Cat#MD31 |
| CellTiter-Glo Viability Assay | Promega | Cat#G924A |
| eBioscience Foxp3 / Transcription Factor Staining Buffer Set | eBioscience | Cat#00-5523-00 |
| TaqMan reverse transcription reagent | Thermo Fisher | Cat#N8080234 |
| SYBR Green PCR master Mix | Thermo Fisher | Cat#4309155 |
| Deposited Data | ||
| RNA sequencing data | This paper | GEO: GSE141684 |
| RNA sequencing data | Ruscetti et al., 2018 | GEO: GSE110397 |
| Mouse whole exome sequencing | This paper | NCBI BioProject: PRJNA607826 |
| Experimental Models: Cell Lines | ||
| PANC-1 (human) | ATCC | Cat#CRL-1469; RRID: CVCL_0480 |
| PU-8988T (human) | DSMZ | Cat#ACC-162; RRID:CVCL_1847 |
| MiaPaca-2 (human) | ATCC | Cat#CRL-1420; RRID:CVCL_0428 |
| 3B11 (murine) | ATCC | Cat#CRL-2160; RRID:CVCL_5487 |
| Pdx1-Cre; LSL-KrasG12D/+; LSL-p53R172H/wt (KPCmut) PDAC cells/organoids (murine) | This paper | N/A |
| Primary pancreatic fibroblasts from C57BL/6 mice | Cell Biologics, Inc. | Cat#C57-6201 |
| Primary BMDM (Bone marrow-derived macrophages) from C57BL/6 mice | This paper | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6 | Jackson laboratory | Cat#JAX:000664; RRID:IMSR_JAX:000664 |
| Mouse: NOD-scid IL2Rγnull: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ | Jackson laboratory | Cat#JAX:005557; RRID:IMSR_JAX:005557 |
| Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J | Jackson laboratory | Cat#JAX:003831; RRID:IMSR_JAX:003831 |
| Mouse: LSL-K-ras G12D: B6.129S4-Krastm4Tyj/J | Jackson laboratory | Cat#JAX:008179; RRID:IMSR_JAX:008179 |
| Mouse: Pdx1-Cre: B6.FVB-Tg(Pdx1-cre) 6Tuv/J | Jackson laboratory | Cat#JAX:014647: RRID:IMSR_JAX:014647 |
| Mouse: LSL-p53R172H: p53LSL.R172H 129svj | Jackson laboratory | Cat#JAX:008652; RRID:IMSR_JAX:008652 |
| Mouse: p53flox: B6.129P2-Trp53tm1Brn/J | Jackson laboratory | Cat#JAX:008462; RRID:IMSR_JAX:008462 |
| Oligonucleotides | ||
| shRNA sequence: Renilla: TAGATAAGCATTATAATTCCT | Chicas et al., 2010 | N/A |
| shRNA sequence: p65: TATAGCTTCAGGGTACTCCATC | Chien et al., 2011 | N/A |
| qPCR primer: p65 left: CTCAACTTCTGTCCCCAAGC | This paper | N/A |
| qPCR primer: p65 right: GAGGTACCATGGCTGAGGAA | This paper | N/A |
| qPCR primer: 36B4 left: GCTCCAAGCAGATGCAGCA | Morris et al., 2019 | N/A |
| qPCR primer: 36B4 right: CCGGATGTGAGGCAGCAG | Morris et al., 2019 | N/A |
| See Table S2 for additional qPCR primers | N/A | N/A |
| Recombinant DNA | ||
| Plasmid: MSCV-Luciferase-IRES-GFP | This paper | N/A |
| Plasmid: LMP-shRNA | Chicas et al., 2010 | N/A |
| Software and Algorithms | ||
| Vevo LAB software | VisualSonic | VevoLab |
| Living Image software | Caliper Life Science | Cat#128113; RRID:SCR_014247 |
| STAR | Dobin et al., 2013 | RRID:SCR_015899; |
| featureCounts | Liao et al., 2014 | RRID:SCR_012919; http://bioinf.wehi.edu.au/featureCounts/ |
| R | N/A | https://www.r-project.org |
| cutadapt | Martin, 2011 | RRID:SCR_011841; http://gensoft.pasteur.fr/docs/cutadapt/1.6/guide.html |
| BWA | Li and Durbin, 2009 | RRID:SCR_010910; http://bio-bwa.sourceforge.net |
| PICARD | N/A | RRID:SCR_006525; http://broadinstitute.github.io/picard/index.html |
| GATK | McKenna et al., 2010 | RRID:SCR_001876; https://gatk.broadinstitute.org/ |
| ABRA | Mose et al., 2014 | RRID:SCR_003277; https://github.com/mozack/abra |
| muTect | Cibulskis et al., 2013 | RRID:SCR_000559; https://software.broadinstitute.org/cancer/cga/mutect |
| DESeq2 | Love et al., 2014 | RRID:SCR_015687; https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| Trimmomatic | Bolger et al., 2014 | RRID:SCR_011848; http://www.usadellab.org/cms/?page=trimmomatic |
| HTSeq | Anders et al., 2015 | RRID:SCR_005514; https://htseq.readthedocs.io/en/release_0.11.1/ |
| Enrichr | Kuleshov et al., 2016 | RRID:SCR_001575; http://amp.pharm.mssm.edu/Enrichr |
| Other | ||
| Vevo 2100 System ultrasound | VisualSonic | RRID:SCR_015816 |
| Xenogen IVIS Spectrum imager | PerkinElmer | Cat#124262 |
| Typhoon FLA 7000 laser scanner | GE healthcare | Cat#28-9558-09 |
| Gentle MACS Octo dissociator with heaters | Miltenyi Biotech | Cat#130-096-427 |
| Guava Easycyte | Millipore | Cat#0500-4008 |
| ViiA 7 Real-Time PCR System | Invitrogen | Cat#4453536 |