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. 2020 May 15;21(10):3486. doi: 10.3390/ijms21103486

Figure 5.

Figure 5

IS dose-dependent NFATc1 ubiquitination and ARNT expression. Raw 264.7 cells with 50 ng/mL of sRANKL were cultured in IS at 0, 100, 500, and 1000 µM for 3 days. (A) NFATc1 ubiquitination on Day 3 depended on the IS level. NFATc1 ubiquitination at lower IS levels (100 µM) is low, compared to that at higher IS levels (1000 µM). Immunoprecipitated NFATc1 is shown as an input. A representative example of three independent experiments is shown. (B) ARNT expression on Day 3 depended on the IS level. Western blot analysis showed that low IS levels (100 µM) increased nuclear ARNT expression, while high levels (>500 µM) inhibited it. Error bars represent mean ± SD (n = 3). * p < 0.05 compared to the control group.