Table 2.

Sperm viability in different treatments and after incubation at 37 °C for 4 h (Experiment 2). Sperm concentration was set at 500 × 10⁶ sperm/mL in all cases.

Treatment	0 h	1 h	2 h	3 h	4 h
PMN + SP	77.9 ± 4.7 1, a	65.2 ± 7.0 1, 2, a	55.8 ± 6.0 2, 3, a	43.2 ± 4.3 3, a	30.5 ± 3.3 4, a
PMN + SP + FMLP	73.9 ± 6.8 1, a	62.5 ± 7.1 1, 2, a	53.6 ± 7.1 2, 3, a	38.7 ± 4.5 3, 4, a	28.9 ± 3.4 4, a
PMN + SP + DMSO	74.9 ± 7.4 1, a	62.3 ± 8.1 1, 2, a	54.7 ± 6.8 2, 3, a	43.0 ± 8.3 3, 4, a	30.7 ± 7.3 4, a
SP + DMSO	74.5 ± 6.1 1, a	64.2 ± 7.0 1, 2, a	55.0 ± 6.8 2, 3, a	43.3 ± 4.8 3, a	31.2 ± 4.9 4, a
SP	77.7 ± 6.3 1, a	68.1 ± 5.3 1, 2, a	55.5 ± 5.8 2, 3, a	45.1 ± 6.5 3, a	28.3 ± 4.8 4, a

Different superscripts (a, b) mean significant (p < 0.05) differences between treatments within a given time point, and different numbers (1, 2) mean significant (p < 0.05) differences between incubation times within a given treatment. Data are shown as mean ± SEM for seven experiments. PMN: polymorphonuclear neutrophils; SP: seminal plasma; DMSO: dimethyl sulfoxide; FMLP: formyl-methionyl-leucyl-phenylalanine.