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. 2020 May 18;21(10):3564. doi: 10.3390/ijms21103564

Figure 2.

Figure 2

Effects of ALS-CSF on the relations of neuronal cells in control- and mutant SOD1-MNs. (a) Left: A significant decrease in the relations of TuJ-1+cell/Hoechst when treated with either control- (* p < 0.05 by one-way ANOVA with Tukey post-hoc test) or ALS-CSF (* p < 0.05 by one-way ANOVA with Tukey post-hoc test), but no significant effect comparing respective CSF conditions. Right: The mutant SOD1-cell line exhibited a significant decrease in the relations of TuJ-1+cell/Hoechst when exposed to ALS-CSF compared to the no CSF condition (# = Kruskal-Wallis test H(2) = 7.636, p < 0.05, z = 2.763, p < 0.05 with Dunn-Bonferroni post-hoc test), but again no significant effect comparing control- with ALS-CSF (29.21% vs. 39.91%, z = 1.279, p = 0.602 with Dunn-Bonferroni post-hoc test). (b) Left: The application of CSF had no significant effect in either cell line analyzing the relation of SMI32+MN/Hoechst in the control-cell line. Right: In SOD1 mutant cells, we detected a significant decrease in the amount of total MNs in relation to all cells (MN/Hoechst) comparing either ALS- or control-CSF-treatment condition and non-treated cells (* p < 0.05 by one-way ANOVA with Tukey post-test). (c) The application of CSF had no significant effect in either cell line analyzing the relation SMI32+MN/TuJ-1+cell. Data are depicted as mean ± SEM.