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. 2020 May 18;21(10):3564. doi: 10.3390/ijms21103564

Figure 4.

Figure 4

No aggregation of disease-relevant proteins after exposition to ALS-CSF. (a–c) ALS-CSF caused no detectable aggregates of TDP-43 (a), FUS (b), or SOD1 (c) when applied to healthy donor-derived MNs. Furthermore, there were no differences of cytoplasmic or nuclear localization of any disease relevant protein and—importantly—no cytoplasmic mislocalization of TDP-43 and FUS, respectively. (d–f) Similar results were detected after applying CSF to the mutant-SOD1-(p.R115G)- and (g–i) mutant-FUS-(p.R521C)-cell-line MNs. Scale bar = 10 µm.