Figure 4.

EPO decreases inflammasome expression and changes mitochondrial apoptotic effectors expression. Renal extract was prepared 24 h after ischemia/reperfusion injury (IRI). Expression levels of NLRP-1, NLRP-3, and cleaved caspase-1 (A) and Bax, Bcl-2, and Bcl-xL (B) were analyzed by Western blot. Quantitative analysis of NLRP-1 (C), NLRP-3 (D), cleaved caspase-1 (E), Bax (F), Bcl-2 (G), and Bcl-xL (H) were performed, with results normalized to β-actin. Data are presented as mean ± SEM. *p < 0.05.