Table 2. Similarity between the S. cerevisiae genes encoding the proteins responsible for glycerol transport and metabolism as in Figure 1, and the corresponding sequences identified in the genome of W. anomalus LBCM1105. Protein Sequences are available at https://doi.org/10.6084/m9.figshare.11441061.v1.
| Protein role | S. cerevisiae - SGD database | Gene | Percentage target aligned | Similarity | |||
|---|---|---|---|---|---|---|---|
| Gene | ID | ||||||
| Regular pathway | Transport | Glycerol channel | FPS1 | S000003966 | g1373.t1 | 45.3 | 56% |
| Glycerol active permease/ H+ symporter | STL1 | S000002944 | g4293.t1 | 85.4 | 57% | ||
| Consumption | Glycerol kinase | GUT1 | S000001024 | g1371.t1 | 91.2 | 72% | |
| Glycerol 3P | GUT2 | S000001417 | g5045.t1 | 98.8 | 72% | ||
| dehydrogenase/mitochondria | |||||||
| Production | Glycerol 3P dehydrogenase | GPD1 | S000002180 | g1302.t1 | 100 | 78% | |
| Glycerol 3P dehydrogenase | GPD2 | S000005420 | g1302.t1 | 81.1 | 82% | ||
| Glycerol 3P phosphatase | GPP1 | S000002180 | g4575.t1 | 99.2 | 71% | ||
| Glycerol 3P phosphatase | GPP2 | S000005420 | g4575.t1 | 99.2 | 71% | ||
| Alternative pathway | Consumption/Production | Glycerol dehydrogenase | GCY1 | S000005646 | g1045.t1 | 98.7 | 79% |
| Glycerol dehydrogenase | YPR1 | S000002776 | g1045.t1 | 98.7 | 78% | ||
| Consumption | Dihydroxyacetone kinase | DAK1 | S000004535 | g4297.t1 | 98.5 | 56% | |
| Dihydroxyacetone kinase | DAK2 | S000001841 | g4297.t1 | 97.8 | 52% | ||