Skip to main content
. 2020 May 14;21(10):3464. doi: 10.3390/ijms21103464

Figure 4.

Figure 4

TDP-43 and FUS-induced misfolded HuWtSOD1 propagates from transfected cells to untreated spinal cord cultures. Primary spinal cord cultures containing neurons (including motor neurons) and astrocytes prepared from human HuWtSOD1 transgenic mice were incubated for 20 h with conditioned media from transfected HEK293 cells, and stained for misfolded SOD1 (green) using misfolded SOD1-specific antibody, 3H1, and counterstained using Hoechst 33342 (blue). The source of the media is indicated for each panel. Primary cultures incubated with conditioned media from FUSR495X, FUSP525L, wtTDP-43, and TDP- 43ΔNLS showed an increase in the presence of cytoplasmic misfolded SOD1 when compared to cells incubated with conditioned media from cells transfected with empty vector control and wtFUS. Scale bar: 75 μm. Figure 4 has been adapted from Grad, L.I., Pokrishevsky, E., Silverman, J.M., and Cashman, N.R. (2014). Exosome-dependent and independent mechanisms are involved in prion-like transmission of propagated Cu/Zn superoxide dismutase misfolding. Prion 8(5), 331–335, 2014. with the permission of Dr. Neil Cashman who is the corresponding author on the paper.